Mansour Amin
Ahvaz Jundishapur University of Medical Sciences, Iran
Title: Detection of Haemophilus influenzae type b, Streptococcus pneumonia and Neisseria meningitides in CSF of children suspected to bacterial meningitis in Ahvaz, Iran
Biography
Biography: Mansour Amin
Abstract
Introduction: Bacterial Meningitis (BM) is a medical emergency and is a life threatening condition in the populations of infants and children. The most common etiologic agents of acute BM are Haemophilus influenzae type b (Hib), Streptococcus pneumoniae and Neisseria meningitides.
Material & Methods: In this study, 196 cerebrospinal fluid samples (CSF) were taken from the infants and children under 5 years of age suspected to BM. Three milliliter of CSF was collected in three sterile tubes. The first tube was used for blood cell count, protein and glucose detection, the second tube was used for bacterial culture and direct smear and the tertiary was used for DNA extraction and PCR.
Results: Out of 196 cases, BM was confirmed by PCR in 9 samples and bacterial culture in 3 samples. The frequency of Hib was 8 cases. Furthermore, Hib was detected in 3 cases by both of the bacterial culture and PCR but in the five other cases, this bacterium was recognized only by PCR. S .pneumoniae was detected in one case only by PCR. In this study, any N. meningitides was not found in the based on the culture or PCR.
Conclusion: The frequency of Hib in Iran is more than other bacterial meningitis agents in Iran. The introducing of Hib vaccine has been started from December 2014 in Iran and the samples of this study were taken before this time. We wish the immunization protect the children and decries the rate of Hib in next studies.