Sun Shin Yi
Soonchunhyang University, Asan31538, Republic of Korea
Title: A suggestion about rapid rational conditions of CNS safety from various vaccine strategy
Biography
Biography: Sun Shin Yi
Abstract
The most realistic way before the recent epidemic that occurred in unexpected times and places in the world is rapid supplying of vaccines related with the infectious diseases within limited times. However, little considerations about rapid supplying a precise manual for the safety assurance of central nervous system(CNS) were established well so far. Thus, this careless in the development of general and/or emergent vaccines should be corrected with a certain secure of safety protocol which can be reduced risks on CNS damages before distribution. Hereby, the present study is undertaken to establish a manual which a certain material can make CNS damaged such as breaking down blood-brain barrier (BBB) protecting brain. Since BBB is critical morphological structure selective permeability between blood vessels and brain, it would be very important to know which conditions (i.e. post-injection, -time) can make BBB vulnerable by pyrogenic inflammatory agent such as Lipopolysaccharide(LPS) systemic injection.
Following IP administration of the LPS to the mice, the mRNA levels of typical markers of the damaged BBB tight junction such as ZO-1 and CLDN 5 were checked out. As conditions in the LPS, IV Evans Blue administration after IP LPS administration according to each concentration (four conditions of concentration) of LPS concentrations. BBB damages were able to be measured by Evans Blue existence checks by fluorescence wavelength ranges from (ex: 620nm /em: 680nm) in the brain tissue. Ultimately, we could observe the mutual relations by comparison with the two methods (mRNA and wavelength levels). According to the results, we set LPS concentration can open BBB and by the mRNA levels of tight junction, we can apply these results to general/emergency vaccine strategy.