Maria Fernanda Chiari graduated in biological sciences at Universidade Estadual do Norte do Paraná (2007) and became master in biotechnology at Universidade Federal de São Carlos in 2010. She is currently a fellow of CAPES doctoral program in biotechnology also from the Universidade Federal de São Carlos - UFSCar, working in the field of parasitology and immunology
Disseminated strongyloidiasis is rare among the population of immunocompetent, but very important in immunocompromised individuals, particularly those co-infected with HIV. This form of the disease is due to autoinfection capacity of Strongyloides stercoralis and characterized by the presence of larvae in multiple organs. The search for new compounds that have antiparasitic activity and less side effects is of great interest for the treatment of immunocompromised individuals. The search for new drugs has an important ally in medicine for centuries, the use of medicinal plants. And in our group we have shown beneficial effects of Mentha piperita L. in the treatment of experimental schistosomiasis. Thus, we evaluated the Menthol and menthone compound in the viability of in vitro parthenogenetic females Strongyloides venezuelensis. After 12 days of infection of rats (Wistar -1500 larvae per animal),15 cm of their small intestine were removed and the females were recovered. Two females per well containing 2 mL medium and compounds and Menthol and / or menthone (100 ug/ml, 50 ug/ml, 25 ug/ml and 12.5 ug/ml) in triplicate. We utilized Ivermectin (the same doses to compounds) to positive control and DMSO 99.9% to negative control. The tested compounds showed 100% efficiency for induction of in vitro female death, suggesting that these bio compounds have potential to be assessed for their effectiveness in reducing the parasitic load in vivo tests. And they can contribute to the search for new compounds in the control of parasitic diseases caused by nematodes
Nada Matougui is a second year PhD student in biology and health doctorate school at the University of Angers working under the supervision of the professor Patrick Saulnier. Her research is focused on the development of a Lipid-based Nanoformulation of Antimicrobial Peptides to Treat Bacterial Infectious Diseases. She is pharmacy graduate from the Medicine University of Algiers, followed by a Master degree in “Technologies Innovantes en Formulation” at University of Angers. She worked as an intern at the “MINT” laboratory on the development of a nanomedicine for glioblastoma therapy.
The rapid increase in drug-resistant infections presents an acute problem that continues to challenge the healthcare sector, generating interest in novel antimicrobial strategies. Antimicrobial peptides (AMPs) have a high potential as new therapeutics against infectious diseases as they are less prone to induce resistance due to their fast and non-specific mechanism of action. The new peptides included in the study are well-defined AMPs, established to have an antimicrobial effect and an acceptable safety profile. The aim of this work is to explore the potential of lipid nanocapsules (LNCs) for AMP delivery, and especially its ability to protect the peptide against degradation while at the same time maintain proper drug activity. The LNCs are described as an oily core composed of medium chain triglycerides, and surrounded by a surfactant shell made of lecithin and PEGylated surfactants. Their lipidic cores are not favorable as they stand to encapsulate hydrophilic molecules. To promote the peptide loading, the incorporation of the cationic peptides in the shell of the LNCs was envisaged. Two strategies are tested: adsorption of the AMPs on the surface of LNCs by incubation under magnetic stirring or incorporation of charged linkers to the formulation of LNCs. The incubation performed at different conditions shows a good association of the peptides to the surface of the LNCs. The minimal inhibitory concentrations (MIC) of the LNC-AMPs were determined for the sensitive strains. The results show a preservation of the antibacterial activity of the native peptide and in some cases a decrease of the MIC.