Jack Ho WONG
Chinese University of Hong Kong, HKSAR
Title: Fungicidal Effect of Bovine Lactoferrin Fragments in Human Pathogenic Fungus (Candida Albicans)
Biography
Biography: Jack Ho WONG
Abstract
In this study, synthetic bovine lactoferricin (Lfcin B) 17-30 and lactoferrampin (Lfampin B) 265-284 were tested for antifungal activity against planktonic Candida albicans strain SC5314. Exposure of fungal cells to Lfcin B 17-30 brought about morphological changes demonstrating the presence of vesicles upon pseudohyphae or hyphae; alterations of plasma membrane permeability; and several hallmarks of apoptosis comprising phosphatidylserine externalization, reactive oxygen species (ROS) production, DNA fragmentation, changes in mitochondrial membrane potential and activation of caspase. From the aspect of gene expression, Lfcin B 17-30 probably brought about ROS accumulation by suppressing the expression of superoxide dismutase 3 (SOD3). In addition, the suppression of gene FRE7, CTR1 and SIT1 may be associated with changes of plasma membrane permeability. The minimal fungicidal concentration of Lfampin B 265-284 was determined to be 77.5μg/ml against C. albicans cells at a concentration of 1x107 cell/ml. Lfampin B 265-284 exerted its antifungal effect mainly through necrosis and apoptosis. Lfampin B 265-284 brought about changes in the fungal membrane permeability and mitochondrial membrane potential and caspase activation. Treatment with Lfampin B 265-284 for 3 hours led to suppression of GPX2 and increase of PXP2, which are both related to oxidative stress.